Instrumentation
- Stratalinker
- MiSeq FGx Sequencer
- iScan Microarray System
- BioRad T100 thermocycler
- Eppendorf thermomixer
- Eppendorf refrigerated thermomixer
- 3x Eppendorf 5415D benchtop centrifuges
- Eppendorf Vacufuge
- BioRad C1000 thermocycler
- Eppendorf 5804 centrifuge + deep well plate rotor
- 2x Eppendorf 5430 Refrigerated Centrifuges
- 2x High Speed Digital Microplate shakers
- PBI barocycler
- Maxwell 16 automated extraction system
- Freedom EVO-Tecan Robotic Liquid Handler
- ABI 310 Genetic Analyzers
- ThermoFisher ABI SeqStudio Genetic Analyzer
- 2x Hybridization ovens (Illumina Microarrays)
- Vacuum dessicator
- Thermal plate sealer
- Qubit 2.0 Fluorometer
- Qubit 4.0 Fluorometer
- Quant Studio 3 High Resolution Melt PCR System
- ProFlex thermocycler (96-well block)
- Eppendorf D30 Biophotometer
- OMAX digital microscope
- Ohaus analytical scale
- 3x laminar flow hoods
Software
- Sequencher (one PC license and one Mac license)
- CLC Genomics workbench (Network license) with Microbial workbench (2 desktop licenses)
- Geneious
- BaseSpace (Illumina) Commercial Subscription
- Illumina Experiment Manager/Sequencing Analysis Viewer
- Illumina GenomeStudio+Methylation
- ExactID
- PrimerE, version 7
- GeneMapper v3.7
Our facility offers a variety of services ranging from case work to validations as well as support for research. Please contact Christina Burns with questions, scheduling and information on pricing.
| Service Description | Unit Base | Internal | External |
| DNA Fragment Analysis | Per sample | $5 | $16 |
| Real-Time PCR (min 8 samples, incl. calib. stds) | Per sample | $14 | $34 |
| Cannabis DNA Typing | Per sample | $44 | $65 |
| Presumptive species ID | Per sample | $13 | $19 |
| Human and Non-human DNA ID | Per sample | $17 | $26 |
| DNA Extraction from environmental samples | Per sample | $18 | $100 |
| DNA Extraction from biological samples | Per sample | $22 | $32 |
| DNA quantification - (min 5 samples, incl 2 stds) | Per sample | $6 | $9 |
| Faculty use I (Instrument hours) | Per 30 minutes | $8 | $19 |
| Faculty use II (Unsupervised bench work) | Per hour | $24 | $35 |
| Faculty use III (Supervised bench work) | Per 30 minutes | $15 | $21 |
| Faculty use IV (Long-term use reservations) | Per lab/day | $41 | $798 |
| Technical training I (Bench training - new users) | Per 30 minutes | $13 | $87 |
| Technical training II (New instrument users) | Per 30 minutes | $6 | $21 |
| Sanger Sequencing (amplicons of 40-800 bp) | Per sample | $10 | $20 |
| NextGen DNA sequencing run (>30 hours) | Per run | $322 | $1123 |
| NextGen DNA sequencing run (up to 30 hours) | Per run | $289 | $617 |
| Specialized Lab Tech Services | Per hour | $30 | $96 |
| NextGen/Microarray Consultation | Per hour | $30 | $44 |
| Project service technician I | Per hour | $12 | $18 |
| Project service technician II | Per hour | $16 | $24 |
| Project service technician III | Per hour | $19 | $28 |
The research conducted at our facility under the supervision of Dr. DeEtta K. Mills focuses on the development of DNA profiling methods for nonhuman organisms such as soil microbial communities, equines, plants, fungi and others.
Ongoing research projects in our facility include:
- Spatial autocorrelation of soil biota profiles with soil type across Miami-Dade County.
- Study of bacterial growth, on bacteria obtained from soil, in the presence of iron and Oxytetracycline.
- Surveillance of tetracycline-resistance genes (tet genes) within antibiotic-resistant soil bacteria communities found in Miami-Dade county soils and comparison across the different soil types to discover diversity of these genes.
- Investigation of the diversity of Vancomycin Genes in Microbes in Sub-Tropical Soils.
- Comparative analysis of freshwater and marine cyanobacteria to determine the role of iron in community virulence and black band disease in corals.
- Major Histocompatibility Complex genes as genetic markers in the conservation and management of wild equids.
- Hyperkalemic periodic paralysis (HYPP) genetic profiling for domestic and wild equine subjects
- Coat color genotyping determination on wild mustangs
- Canine olfaction for the detection of pathogens such as Raffaelea lauricola in laurel wilt diseased avocado trees
